RNAseq of Ogataea polymorpha NCYC495 : MATa/alpha diploid in YPD : re... - SRA

SRX3393942: RNAseq of Ogataea polymorpha NCYC495 : MATa/alpha diploid in YPD : replicate 2
1 ILLUMINA (Illumina HiSeq 2500) run: 20.6M spots, 1G bases, 666.2Mb downloads

Design: To examine the transcriptional response induced by nitrogen depletion, we used mRNAseq to compare the transcriptomes of O. polymorpha cells 2 h after transfer from a YPD pre-induction culture into NaKG, to parallel cultures transferred into fresh YPD. Furthermore, because we expect that switching occurs only in haploid cells, we conducted this experiment in parallel on haploid (MATa and MATα isogenic strains) and diploid (MATa/α) cells.

Submitted by: University College Dublin

Study: RNAseq to examine the transcriptional response induced by nitrogen depletion in Ogataea polymorpha

PRJNA418110 • SRP124832 • All experiments • All runs

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RNAseq to examine the transcriptional response induced by nitrogen depletion in Ogataea polymorpha for "Flip/flop mating-type switching in the methylotrophic yeast Ogataea polymorpha is regulated by an Efg1-Rme1-Ste12 pathway" PLOS Genetics (2017), In Press. Hanson SJ, Byrne KP & Wolfe KH.

Sample: RNAseq of Ogataea polymorpha NCYC495 : MATa/alpha diploid in YPD

SAMN08014809 • SRS2680254 • All experiments • All runs

Organism: Ogataea polymorpha

Library:

Name: Opol_Mating_MATaalpha_YPD_R2

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: SINGLE

Spot descriptor:

1 forward

Runs: 1 run, 20.6M spots, 1G bases, 666.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR6292831	20,639,546	1G	666.2Mb	2017-11-15

ID:: 4733455

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